Composite

Part:BBa_K2066022

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-08-29)


Constitutive tetR + UNS

This is a composite part with a constitutive Anderson promoter J23105 to express TetR repressor. Part used to express the repressor TetR that can bind to pTET (promoter) to block the expression of GFP (or any gene that pTET controls). The tetR repressor protein can be temporarily blocked by aTc induction to allow for transcription of gene product controlled by pTET.

This part is on the UNS backbone standard developed by William and Mary iGEM 2016, for ease of cloning via Gibson or Golden Gate Assembly. The insert is flanked by UNS 2 (BBa_K2066018) and UNS 3 (BBa_K2066019) sequences.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 47
    Illegal NheI site found at 70
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None